Metabolism Protein
About protein metabolism in biochemistry?
Protein are high molecular weight polymers of a-amino acids, joined together with peptide linkage, composed of C, H, N and some contain sulphur and phosphorous. Protein are chief constitute of all living cells and play various physiological roles.
1. what is transamination?
2. what is oxidative deamination?
3. what is the importance of urea synthesis in humans?
4. what is the test method to detect the presence of tryptophan in a protein solution and what is its outline?
1>The transfer of an amino (-NH2)group from an amino acid to a keto acid is known as transamination,catalyzed by a group of enzymes called transaminase(aminotransferase)
2>The removal of amino group from the amino acids as NH3 is deamination,it results in the liberation of ammonia for urea synthesis.
3>The nitrogen of amino acids ,converted to ammonia is toxic to the body .Its converted to urea and detoxified .urea is then excreted in urine.
4>there are two test xanthoprotein and millon’s test.
>Millon’s test=Tryptophan when reacts with millon’s reagent produces a tan colored ppt.
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